Seegene multiplex PCR tests and All in One automation

Efficient and comprehensive identification of infectious diseases with automated multiplex PCR method

With Seegene’s unique technologies (DPO, TOCE, MuDT, 3 Ct) and flexible All-in-One automation, molecular diagnostics of infectious diseases reach unparalleled performance, scope, and efficiency. Seegene offers a range of test panels that can be customized by combining high-quality clinical diagnostic solutions for various needs and sample materials.

  • DPO™ PCR Primers: Achieve the sensitivity of a single PCR reaction in multiplex reactions.
  • TOCE™ Technology: Enables melting curve analysis of amplification products with single-nucleotide precision, allowing differentiation of genetically similar pathogens. This technology supports the identification of multiple targets from a single tube.
  • MuDT™ Technology: Allows the detection of two different target genes with Ct values from one fluorescence channel.
  • 3 Ct PCR™ Technology: Builds on previous Seegene technological innovations to differentiate up to three different target genes from one fluorescence channel, enabling the detection of up to 15 target genes from a single tube.

All in one automation solutions for sample preprocessing, nucleic acid extraction, and PCR pipetting

Seegene provides equipment for tube decapping, nucleic acid extraction, and PCR pipetting, as well as PCR amplification. All test panels can be integrated into a single device, and the same extraction protocol and workflow apply to all sample types, enhancing usability and flexibility. The Seegene Launcher software ensures smooth transitions between stages and seamless sample data transfer across different phases. The Seegene Viewer software used for result interpretation provides semi-quantitative results with Ct values in addition to microbial identification.

Instruments and multiplex PCR assays

Identification of multiple respiratory pathogens by combining various Allplex™ test panels.

  • Targets Include: 20 viruses and 7 bacteria such as Influenza A virus/A-H1/H1pdm09/A-H3, Influenza B virus, Respiratory syncytial virus (RSV) A/B, Adenovirus, Enterovirus, Metapneumovirus, Parainfluenza virus 1/2/3/4, Bocavirus 1/2/3/4, Coronavirus 229E/NL63/OC43, Human rhinovirus, Bordetella pertussis, Bordetella parapertussis, Chlamydophila pneumoniae, Haemophilus influenzae, Legionella pneumophila, Mycoplasma pneumoniae, Streptococcus pneumoniae, and SARS-CoV-2.
  • Subtyping: Differentiates subtypes of Influenza A.
  • Virus differentiation: Distinguishes between rhinovirus and enterovirus.
  • Virus separation: Differentiates SARS-CoV-2, Influenza A, Influenza B, and RSV.
  • Suitable sample materials: Include upper respiratory tract swabs and wash samples, bronchoalveolar lavage, and sputum.

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Identification of multiple gastrointestinal pathogens by combining various Allplex™ test panels.

  • Coverage includes: 6 viruses, 14 bacteria, and 15 parasites/intestinal worms.
  • Hypervirulence markers: Identifies several hypervirulence markers.
  • Targets include: Aeromonas spp., Campylobacter spp., Clostridium difficile toxin B, Salmonella spp., Shigella spp./EIEC, Vibrio spp., Yersinia enterocolitica, EAEC, EPEC, ETEC, Escherichia coli O157, Hypervirulent Clostridium difficile, STEC, Blastocystis hominis, Cryptosporidium spp., Cyclospora cayetanensis, Dientamoeba fragilis, Entamoeba histolytica, Giardia lamblia, Adenovirus, Astrovirus, Norovirus GI/GII, Rotavirus, Sapovirus, Ancylostoma spp., Ascaris spp., Enterobius vermicularis, Enterocytozoon spp./Encephalitozoon spp., Hymenolepis spp., Necator americanus, Strongyloides spp., Taenia spp., Trichuris trichiura, Helicobacter pylori.
  • Suitable sample materials: Include stool, stool or rectal swab, and gastrointestinal biopsy.

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Identification of multiple sexually transmitted pathogens by combining various Allplex™ test panels.

  • Detection Includes: 17 bacteria, 4 viruses, and 7 fungal species.
  • Targets include: Atopobium vaginae, Bacterial vaginosis–associated bacteria 2, Bacteroides fragilis, Gardnerella vaginalis, Lactobacillus spp., Megasphaera Type 1, Mobiluncus spp., Candida albicans, Candida dubliniensis, Candida glabrata, Candida krusei, Candida lusitaniae, Candida parapsilosis, Candida tropicalis, Cytomegalovirus, Haemophilus ducreyi, Herpes simplex virus 1/2 (HSV1, HSV2), Lymphogranuloma venereum, Treponema pallidum, Varicella zoster virus, Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Mycoplasma hominis, Trichomonas vaginalis, Ureaplasma parvum, Ureaplasma urealyticum.
  • Screening test: Includes a CT/NG/MG/TV combination test and follow-up testing for resistance genes (NG, MG).
  • Suitable sample materials: Include genital or cervical swabs, urine, and liquid cytology samples.

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Allplex™ HPV HR screening test and Allplex™ HPV28 genotyping test aid in cervical cancer screening.

  • Screening test (HPV HR): Differentiates 14 high-risk HPV genotypes (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68).
  • Genotyping test (HPV28): Differentiates 19 high-risk (16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 69, 73, 82) and 9 low-risk HPV genotypes (6, 11, 40, 42, 43, 44, 54, 61, 70).
  • Suitable sample materials: Include liquid cytology samples, and genital or cervical swabs.

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Identification of bacterial or viral meningitis by combining various Allplex™ test panels.

  • Detection includes: 12 viruses and 6 bacteria.
  • Targets include: Escherichia coli K1, Group B Streptococcus (GBS), Haemophilus influenzae, Listeria monocytogenes, Neisseria meningitidis, Streptococcus pneumoniae, Adenovirus, Enterovirus, Human parechovirus, Mumps virus, Parvovirus B19, Cytomegalovirus, Epstein-Barr virus, Herpes simplex virus 1/2/6/7 (HSV), Varicella zoster virus.
  • Suitable sample material: Cerebrospinal fluid.

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Simultaneous detection of antibiotic resistance genes in Gram-positive and Gram-negative bacteria using the Allplex™ Entero-DR Assay.

  • Detection includes: 8 carbapenemase, vancomycin, and ESBL resistance genes.
  • Targets include: CTX-M, Imipenemase (IMP), Klebsiella pneumoniae carbapenemase (KPC), New Delhi Metallo-beta-lactamase (NDM), Oxacillinase-48 (OXA-48), VanA, VanB, Verona Integron-encoded Metallo-ß-lactamase (VIM).
  • Suitable sample materials: Rectal swab and bacterial colony.

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Detection of Mycobacterium tuberculosis (MTB), the bacterium causing tuberculosis, and its characteristic resistance genes using various Allplex™ tests.

  • Targets include: Isoniazid resistance (7 mutations), Rifampicin resistance (18 mutations), Fluoroquinolone resistance (7 mutations), Injectable drug resistance (6 mutations)
  • Suitable sample materials: Sputum, cell culture, and bronchoalveolar lavage samples.

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Seegene STARlet-AIOS™

This fully automated system integrates pipetting automation and PCR into a single unit. The entire workflow, from sample to result, is conducted within the device, with softwares integrated into a single control computer. STARlet-AIOS is an excellent tool for high-capacity studies and extensive automation needs.

  • Maximum capacity: 188 tests per run and 752 tests per day, or more, depending on the number and combination of test panels used.
  • Time to first results: Less than 5.6 hours.
  • Batch loading of samples: Allows samples to be loaded in batches.

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Seegene STARlet IVD

An automation device for nucleic acid extraction from various primary sample tubes and for PCR pipetting.

  • Capacity: Processes 94 samples in 155 minutes.
  • Benefits: Enhances method reliability and frees up resources by automating workflow steps.
  • Flexibility: Allows different tests to be combined on the same platform.

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VCMS

The Vial Cap Management System (VCMS) is a preprocessing device that automates the removal of caps from liquid cytology sample tubes before HPV testing and resealing after sample pipetting. The device can be directly integrated with the Seegene Starlet IVD device and workflow.

  • Capacity: Processes 92 Thinprep™ tubes in a single run.
  • Purpose: Enhances workflow construction and maximizes automation.

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Nimbus IVD

An automation device for nucleic acid extraction from various primary sample tubes and for PCR pipetting.

  • Capacity: Processes 72 samples in 180 minutes.
  • Flexibility: Allows different tests to be combined on the same platform.

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SEEPREP32™

A compact automation device for nucleic acid extraction.

  • Capacity: Processes 32 samples in 30 minutes.
  • Capability: Combines multiple different sample materials in the same extraction.
  • Technology: Utilizes magnetic bead-based extraction.

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CXF96™ Dx real-time PCR System

A real-time PCR device combined with Seegene Viewer software ensures comprehensive multiplex results.

  • Capacity: Processes 96 samples.
  • Features: Includes 5 different fluorescence channels.

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Product is available in the following countries:

  • Finland

Links and documents

Seegene All-In-One Platform

Manufacturer’s website

Manufacturer’s COVID-19 page

Manufacturer’s YouTube channel

Keywords

liquid handling PCR

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